By M. Cristina Vega
This booklet offers complex expression applied sciences for the creation of protein complexes. for the reason that complexes lie on the center of recent biology, the expression, purification, and characterization of enormous quantities of high quality protein complexes is essential for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and bug cells to advanced reconstitution from person subunits, this booklet deals worthwhile insights and assistance for winning protein expressionists.
Across a number of sections readers will observe present possibilities for the construction of protein complexes in bacterial structures (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, crops and algae. advanced reconstitution from separately purified subunits or subcomplexes is mentioned as a complementary approach. a final part introduces in short many of the biophysical and structural characterization innovations for macromolecular complexes utilizing state of the art answer scattering and nuclear magnetic resonance.
This paintings is a guided journey over probably the most robust and profitable protein expression applied sciences, with a spotlight on co-expression and high-throughput functions. it's addressed to all people drawn to the construction and characterization of macromolecular complexes, from collage scholars who wish an obtainable description of the foremost co-expression structures to researchers in biomedicine and the existence sciences looking for an updated survey of accessible technologies.
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Extra info for Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology)
Advanced Technologies for Protein Complex Production and Characterization (Advances in Experimental Medicine and Biology) by M. Cristina Vega